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DETERMINATION OF LC50 OF CHLORPYRIFOS AND NEEM EXTRACT
ON THIRD INSTAR LARVAE OF HOUSE FLIES
AND THEIR EFFECT ON FECUNDITY
Islam Dad, Masarrat Jahan Yousuf* and Syed Ishtiaq Anjum
Department of Zoology, University of Karachi, Karachi, Pakistan
Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan
ABSTRACT
Two pesticides one from OP compound (Chlorpyrifos) and other from Biopesticides (Neem extract) were applied against
3rd instar larvae of house flies by contact method. LC50 of Chlorpyrifos and Neem extract was calculated as 0.25% for
chlorpyrifos and 0.42% for Neem Extract. Treated larvae when succeeded to reach the adult stage, the effects of the
mentioned insecticides were noted on fecundity of adults of Musca domestica. Chlorpyrifos delayed egg-laying while
Neem extract completely inhibited the egg-laying. Moreover, the treated larvae when succeeded to pupate, they failed to
emerge from the pupal case completely and the larvae got heavy melanization after 48 hours of treatment.
Keywords: Neem Extract, Chlorpyrifos, Fecundity, Musca domestica.
